American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is using PCR to detect the presence of the mecA gene in Staphylococcus aureus isolates. The mecA gene encodes for:

A) An aminoglycoside-modifying enzyme
B) Beta-lactamase enzyme
C) Altered penicillin-binding protein (PBP2a)
D) An efflux pump protein

2. A patient with a history of solid organ transplantation develops diarrhea and abdominal cramps. Stool studies are negative for bacterial pathogens and C. difficile toxins. Colonoscopy reveals characteristic cytomegalic inclusion bodies in colonic biopsies. The MOST likely causative agent is:

A) Rotavirus
B) Cytomegalovirus (CMV)
C) Adenovirus
D) Norovirus

3. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Modification of the ribosomal target site, preventing antibiotic binding.
B) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
C) Production of an enzyme that inactivates the fluoroquinolone molecule.
D) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.

4. A clinical microbiology laboratory is using isothermal nucleic acid amplification techniques for rapid detection of Mycobacterium tuberculosis in sputum. Compared to conventional PCR, isothermal methods offer the advantage of:

A) Higher sensitivity and specificity for detecting low levels of target DNA.
B) Easier integration with downstream analysis techniques such as Sanger sequencing.
C) Faster reaction times and the ability to be performed without sophisticated thermocyclers.
D) The ability to simultaneously amplify multiple targets in a single reaction.

5. Chagas disease, characterized by acute Romana's sign and potential chronic cardiac and gastrointestinal complications, is caused by which vector-borne protozoan parasite?

A) Toxoplasma gondii
B) Leishmania donovani
C) Trypanosoma brucei gambiense
D) Trypanosoma cruzi

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